IL-31 ELISA Kit

Catalog No. ABIN5026938

Human IL-31 ELISA Kit Colorimetric assay for quantification of Human IL-31.

Quick Overview for IL-31 ELISA Kit (ABIN5026938)

Target: IL-31 (IL31) (Interleukin 31 (IL31))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 7.8-500 pg/mL

Application: ELISA

Sample Type: Plasma, Serum

Product Details IL-31 ELISA Kit

Minimum Detection Limit: 7.8 pg/mL

Purpose: This immunoassay kit allows for the in vitro quantitative determination of Human IL-31 concentrations in serum, plasma and other biological fluids.

Analytical Method: Quantitative

Sensitivity: < 4.69 pg/mL

Components: plate, standard, diluent, antibodies

Material not included: pipettes, tubes, reader, incubator

Application Details

Sample Volume: 100 μL

Assay Time: 3 - 4 h

Plate: Pre-coated

Protocol: This assay employs an antibody specific for Anti-humanIL-31 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-31 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-humanIL-31 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-31 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Restrictions: For Research Use only

Handling

Precaution of Use: protect your eyes

Storage: 4 °C

Target Details for IL-31

Target: IL-31 (IL31) (Interleukin 31 (IL31))

Alternative Name: IL-31 (Interleukin-31)